Alcian Blue Stain (pH 1.0)

Assay-kits

Alcian Blue Stain (pH 1.0)

Product code: ACMBS-30
Specifications: 2x20mL, 2x100mL (Box)
Intended use: Intended for containing sulfated mucus material staining
Store temperature: 2℃～8℃
Expiry date: 18 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

Alcian blue is the most specific stain to detect acid mucous substances. This anion dye forms an electrovalent bond with acid group. Alcian blue can be used to separate and identify different mucous substances at various pH. At pH 1, carboxyl group (COOH) was not stained, sulfonic group (SO₃H) is stained, and at pH 2.5, carboxyl group is well stained, but poor stain of sulfate mucus.

Main components

Reagent composition	Main ingredient
1. Alcian blue solution (pH 1.0)	Alcian blue
2. Hydrochloric acid solution	Hydrochloric acid

Sample requirement

The tissue section must be fully fixed.

Test procedure

1. The slices are routinely dewaxed to water and washed once with distilled water.
2. Place in Alcian blue solution (pH 1.0) for 20～30 minutes.
3. Rinse briefly with hydrochloric acid solution.
4. Without rinse with water, blotted dry with filter paper for the excess hydrochloric acid solution.
5. Conventional dehydration and transparent, sealed with neutral resin.

Result interpretation

Contain sulfated mucilage substance	Blue
Non-sulfated acid mucilage substance	No coloring
Nuclei and other tissues	No coloring

Precautions

1. After removing the reagent from the refrigerator, please wait until it is fully restored to room temperature before use.
2. The Alcian blue solution is a strong stain with stable color. Overstaining is not a concern even after long staining.
3. In test procedure, after Hydrochloric acid solution slightly washed, no longer water rinse, otherwise it will change the pH of the stain solution, causing non-specific staining.
4. This method is not containing counterstain solution, if you need to counterstain the nucleus, you can use nuclear fast red solution (option). After counterstaining, then dehydrate and clear and mounting.
5. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
6. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
7. The production date, production batch number and expiration date are shown in the outer packaging.

References

1. Chinese Medical Association. Clinical technical operating specifications-pathology volumes [M]. People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

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