Metachromatic Granules Stain (Albert Method)

Assay-kits

Metachromatic Granules Stain (Albert Method)

Product code: ACMBS-62
Specifications: 20mL (Box)
Intended use: Mainly for the analysis of corynebacterium diphtheria staining
Store temperature: 5℃～30℃
Expiry date: 24 Months

More Details

PrincipleMain componentsTest procedureResult interpretationMethod limitationPrecautions

Principle

The size of Corynebacterium diphtheriae is very variable, essentially rod-shaped but often showing irregular expansion at one end. Cells frequently lie in small clusters at acute angles to each other in V, L or Y shapes. Gram positive but readily decolorized, non-motile, non-capsulate, non-spore. Strains are distributed as beans and stained unevenly. With Metachromatic Granules Stain, deeply stained granules known as metachromatic granules, are found on one side or both sides of diphtheriae.

Main components

Reagent composition	Main ingredient
1. Solution A	Toluidine blue
2. Solution B	Potassium iodide

Test procedure

1. Fix smears on flames, add solution A to stain for 3～5 minutes and rinse with water.
2. Add solution B to stain for 1 minute and rinse with water.
3. Dry and observe microscopically.

Result interpretation

Corynebacterium diphtheria	Green
Metachromatic granules	Blue-black

Method limitation

Only used for staining of Corynebacterium diphtheria.

Precautions

1. This kit must be used by professionals.
2. Should carefully read the instruction manual before using. Can only be used before expiration date, and good personal hygiene protection.
3. After use, disposal of waste should comply with the hospital or the environmental protection department requirements.
4. Production lot number and expiration date on the package.

References

1. The State Health and Family Planning Commission of the People's Republic of China Medical Administration Bureau. National Clinical Laboratory Procedures [M]. 4th ed. Beijing: People's Medical Publishing House, 2014.

2. Gradwohl's Clinical Laboratory Methods And Diagnosis, 8th, ed.

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