Leukocyte Peroxidase Stain (POX) (Benzidine Method)

Leukocyte Peroxidase Stain (POX) (Benzidine Method)
Leukocyte Peroxidase Stain (POX) (Benzidine Method)

Assay-kits

Leukocyte Peroxidase Stain (POX) (Benzidine Method)

  • Product code: ACMBS-43
  • Specifications: 5Tests, 20Tests, 100Tests (Box)
  • Intended use: This kit is for staining bone marrow cell and blood cell smear
  • Store temperature: 2℃~8℃
  • Expiry date: 12 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationMethod limitationPrecautions

Principle

Benzidine method: The Intracellular peroxidase transferred hydrogen atom of colorless diaminobenzidine to the hydrogen peroxide. the former into a colored dye deposited in the cytoplasm of the enzyme where the parts.

Main components

Reagent compositionMain ingredient
1. Benzidine solution (solution A)Benzidine
2. H2O2 solution (solution B)H2O2
3. Wright-Giemsa solution (solution C)Wright-Giemsa
4. Phosphate buffer (solution D)Phosphate

Sample requirement

  • Fresh bone marrow cell smear and blood cell smear.

Test procedure

  • 1. Add solution A few drops into fresh smear (Cover the smear is appropriate), then add solution B few drops (solution A: solution B=1:1), after mix then stand room temperature 5~10 minutes, then running water flushing 2 minutes.
  • 2. Wright-Giemsa stain: solution C and solution D mixing (C:D=1:2), then staining 3~5 minutes, then running water flushing, after dry go to microscopy.

Result interpretation

  • Positive reaction appeared Yellow-brown or Black-blue granules.
  • Reference range
  • 1. Granulocyte System: Neutrophils other than myeloblasts and cells at lower stages are positive. Eosinophils are found to be stained fast and deeply, basophil are in negative reaction.
  • 2. Monocytes System: Some cells are with weakly positive reaction and have small granules diffusely spread in cells. Some cells are in negative reaction.
  • 3. Macrophages are in positive reaction at variant degrees.
  • 4. Lymphocytes, plasmacytes, nucleated red blood cells, and megakaryocytes are negative.

Method limitation

  • Only use for morphological staining to observe.

Precautions

  • 1. The methodology is the ICSH recommendation method.
  • 2. The kit should be used before the room temperature, shake the reagent before use.
  • 3. Tightly cap the reagent bottle immediately after use to avoid evaporation.
  • 4. This kit must be used by professionals and the interpretation of results.
  • 5. Should carefully read the instruction manual using. Can only be used before expiration date, and good personal hygiene protection.
  • 6. Wastes should be disposed with care as biohazardous materials by following local EPA guidelines.
  • 7. Production lot number and expiration date on the package.

References

1. National Health and Family Planning Commission of the People's Republic of China Medical Administration Hospital Authority. National Clinical Laboratory Procedures [M]. 4th ed. Beijing: People's Medical Publishing House, 2014.

2. Gradwohl's Clinical Laboratory Methods And Diagnosis, 8th, ed.

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