PanGreen™ Universal SYBR^® Green Master Mix

PanGreenTM Universal SYBR^® Green Master Mix is a 2x concentrated, ready for use Master Mix reaction enhanced for dye-based quantitative PCR (qPCR) and compatible with the majority of commercially available real-time PCR systems (ROX-independent and ROX-dependent). It contains NanoTaq hot-start DNA polymerase, dNTPs, MgCl₂, SYBR^® Green I dye, enhancers, stabilizers and essentials for a success PCR reaction.

Kit Contents

• Enhanced nano complex-mediated hot start enzyme DNA polymerase.
• Maximizes product yield with nano technology.
• Nonspecific bands are eliminated during reaction.
• Compatible with the majority of qPCR systems.

1. Thaw PanGreen™ Universal SYBR^® Green Master Mix and the rest of frozen reaction components to a temperature of 4°C. In order to entirely collect solutions, combine thoroughly and centrifuge briefly, then store at 4°C and avoid from light.

2. Prepare (on ice or at room temperature) enough assay Master Mix for all reactions by adding all necessary components, except the DNA template, according to the recommendations in Table 1. View More↘

3. Combine the assay Master Mix thoroughly to ensure consistency and equally dispense the solution into each qPCR tube or into the wells of a qPCR plate. Employ good pipetting practice to ensure assay precision and accuracy.

4. Add DNA samples (and DNase-free H2O if needed) to the PCR tubes or wells containing assay Master Mix (Table 1), seal the tubes or wells with flat caps or optically transparent film. Note: to ensure thorough mixing of reaction components, vortex for approximately 30 seconds (or more).

5. Spin the tubes or plate to remove any air bubbles and collect the reaction mixture in the vessel bottom.

6. Setup the thermal cycling protocol on a real-time PCR instrument according to Table 2.

(Note: optimization may be needed for better performance).

7. Load the PCR tubes or plate into the real-time PCR instrument and commence the run.

8. Perform data analysis according to the instrument-specific instructions.

• Process in the thermal cycler for 35-45 cycles in Table 2. View More↘

• A compatible real-time PCR instrument
• Vortex or equivalent
• Microcentrifuge
• Plates and seals for your instruments

A 500 bp human genomic DNA target was used to compare the two leading competing qPCR Master Mixes. All amplifications were performed in accordance with the manufacturer's instructions. PanGreen™ Universal SYBR^® Green Master Mix exhibited to be effective (mean Ct : 21.29) and highly specific because no second amplification signal could be identified with the melting curve. The results show that it has the best performance as compared with other qPCR Master Mix suppliers.

Figure 1. Performance of PanGreen™ Universal SYBR^® Green Master Mix