PanGreen™ Universal SYBR® Green Master Mix

PanGreen™ Universal SYBR Green Master Mix
PanGreen™ Universal SYBR Green Master Mix
PanGreen™ Universal SYBR® Green Master Mix

Catalog numberQSD01-0100

Size100 reactions (20 μl vol)


StorageStable for up to 3 months at 4°C. Stable for up to 24 months at -20°C.


PanGreenTM Universal SYBR® Green Master Mix is a 2x concentrated, ready for use Master Mix reaction enhanced for dye-based quantitative PCR (qPCR) and compatible with the majority of commercially available real-time PCR systems (ROX-independent and ROX-dependent). It contains NanoTaq hot-start DNA polymerase, dNTPs, MgCl2, SYBR® Green I dye, enhancers, stabilizers and essentials for a success PCR reaction.

Kit Contents

2X Universal SYBR® Green Master Mix 1ml x 1 vial
  • Enhanced nano complex-mediated hot start enzyme DNA polymerase.
  • Maximizes product yield with nano technology.
  • Nonspecific bands are eliminated during reaction.
  • Compatible with the majority of qPCR systems.
Reaction Setup

1. Thaw PanGreen™ Universal SYBR® Green Master Mix and the rest of frozen reaction components to a temperature of 4°C. In order to entirely collect solutions, combine thoroughly and centrifuge briefly, then store at 4°C and avoid from light.

2. Prepare (on ice or at room temperature) enough assay Master Mix for all reactions by adding all necessary components, except the DNA template, according to the recommendations in Table 1. View More↘

Table 1. Reaction Setup

ComponentsVolume per 20 μl ReactionVolume per 10 μl ReactionFinal Concentration
PanGreen™ Universal SYBR® Green Master Mix10 μl5 μl1x
Forward and reverse primersVariableVariable300-500 nM each primer
DNA template (add at step 4) VariableVariablecDNA: 1 pg-10 ng
Genomic DNA: 50 ng-250 ng
Nuclease-free H2OVariableVariable
Total reaction setup volume20 μl10 μl

Note: Optimization may be needed for better performance.

3. Combine the assay Master Mix thoroughly to ensure consistency and equally dispense the solution into each qPCR tube or into the wells of a qPCR plate. Employ good pipetting practice to ensure assay precision and accuracy.

4. Add DNA samples (and DNase-free H2O if needed) to the PCR tubes or wells containing assay Master Mix (Table 1), seal the tubes or wells with flat caps or optically transparent film. Note: to ensure thorough mixing of reaction components, vortex for approximately 30 seconds (or more).

5. Spin the tubes or plate to remove any air bubbles and collect the reaction mixture in the vessel bottom.

6. Setup the thermal cycling protocol on a real-time PCR instrument according to Table 2.

(Note: optimization may be needed for better performance).

7. Load the PCR tubes or plate into the real-time PCR instrument and commence the run.

8. Perform data analysis according to the instrument-specific instructions.

  • Process in the thermal cycler for 35-45 cycles in Table 2. View More↘

Table 2. Thermal Cycling Protocol

Initial Denaturation3-5 minutes at 95°C (5 mins for GC rich or complex templates)
Denaturation15 seconds at 95°C
Annealing & Extension60 seconds at 60°C and Plate Read
Melting curveRefer to specific guidelines for instrument used

Note: Optimal conditions for amplification will vary depending on the primers and thermal cycler used. It may be necessary to optimize the system for individual primers, template, and thermal cycler.

Important notes:

  • Shake gently before use to avoid foaming and low-speed centrifugation.
  • During operation, always wear a lab coat, disposable gloves, and protective equipment.
Required Materials but Not Provided
  • A compatible real-time PCR instrument
  • Vortex or equivalent
  • Microcentrifuge
  • Plates and seals for your instruments

A 500 bp human genomic DNA target was used to compare the two leading competing qPCR Master Mixes. All amplifications were performed in accordance with the manufacturer's instructions. PanGreen™ Universal SYBR® Green Master Mix exhibited to be effective (mean Ct : 21.29) and highly specific because no second amplification signal could be identified with the melting curve. The results show that it has the best performance as compared with other qPCR Master Mix suppliers.

Performance of PanGreen™ Universal SYBR® Green Master MixFigure 1. Performance of PanGreen™ Universal SYBR® Green Master Mix

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