Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)

Catalog numberPDR05-0100

CategoriesMbeads Based

Size100 Reactions

Sample4 rxns ($21.6)

$369.6 Inquire

Note: PureDireX / Purification Kits / Extraction Kit / DNA / Blood / Cultured Cell / Tissue

Description

The Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)(Reagent Based) is a reagent system kit. The kit is designed specifically for genomic DNA isolation from the whole blood, frozen blood, buffy coat, cultured animal/bacterial cells, fungus cells and tissue. This unique reagent system ensures genomic DNA with high yield and good quality from samples. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified genomic DNA is suitable for use in PCR or other enzymatic reactions.

Kit Contents

Contents	NA022-0010
Buffer BR	100 ml
Buffer BC	35 ml
Buffer BP	12 ml

Features

Delivering high-quality genomic DNA with the fast procedure.
Ready-to-use gnomic DNA for high performance in any downstream application.
Highly purified and high yield genomic DNA can be extracted from various samples.
Optimized lysis buffer for the efficient lysis.
Designed to rapidly purify high-quality DNA using spin reagent format.

Application

Gene cloning
Southern blotting

PCR
SNP genotyping

Protocol

Fresh whole Blood or Buffy Coat
Cultured Mammalian Cells
Gram-Negative Bacterial Cells

Gram-Postive Bacterial Cells
Fungus Cells

Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)

Animal Tissue

Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)

Required Materials

Microcentrifuge tubes
Isopropanol
RNase A (10 mg/ml)

TE Buffer
For the tissue sample: Proteinase K(10 mg/ml), Micropestle

Troubleshooting

Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

Problem	Cause	Solution
Low yield of DNA	Incomplete lysed sample	Use the appropriate method for the lysate preparation based on the amount of the starting materials.
		Be sure to add Proteinase K during lysis.
		Increase the digestion time or amount of Proteinase K used for lysis.
		For tissues, cut the tissue into smaller pieces and ensure the tissue is completely immersed in the Lysis step to obtain optimal lysis.
DNA degrade	Sample not fresh	Avoid repeated freeze / thaw cycles of the sample.
	Sample not fresh	Use a new sample for the DNA isolation. Perform the extraction of the fresh material when possible.The yield and quality of DNA isolated is dependent on the type and age of the starting material.
	Inappropriate sample storage conditions	Store mammalian tissues at -80°C and bacteria at -20°C until use. The whole blood can be stored at 4°C for no longer than 1-2 days.
	DNase contaminant	Use the fresh TAE or TBE electrophoresis buffer
	DNase contaminant	Maintain a sterile work environment to avoid contamination from DNases.
Presence of RNA	RNA contamination	Perform RNase A digestion step during the Step Lysis.
Inhibition of downstream enzymatic reactions	Presence of ethanol in purified DNA	Before the DNA Rehydration step, ensure the ethanol was removed completely

Caution

During operation, always wear a lab coat, disposable gloves, and protective equipment.
Check buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.
Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.

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