Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)

Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)
Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)
Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)

Catalog numberPDR05-0100

CategoriesMbeads Based

Size100 Reactions

Sample4 rxns ($21.6)

$369.6 Inquire

Note: PureDireX / Purification Kits / Extraction Kit / DNA / Blood / Cultured Cell / Tissue


The Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)(Reagent Based) is a reagent system kit. The kit is designed specifically for genomic DNA isolation from the whole blood, frozen blood, buffy coat, cultured animal/bacterial cells, fungus cells and tissue. This unique reagent system ensures genomic DNA with high yield and good quality from samples. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified genomic DNA is suitable for use in PCR or other enzymatic reactions.

Kit Contents

Buffer BR100 ml
Buffer BC35 ml
Buffer BP12 ml
  • Delivering high-quality genomic DNA with the fast procedure.
  • Ready-to-use gnomic DNA for high performance in any downstream application.
  • Highly purified and high yield genomic DNA can be extracted from various samples.
  • Optimized lysis buffer for the efficient lysis.
  • Designed to rapidly purify high-quality DNA using spin reagent format.
  • Gene cloning
  • Southern blotting
  • PCR
  • SNP genotyping
  • Fresh whole Blood or Buffy Coat
  • Cultured Mammalian Cells
  • Gram-Negative Bacterial Cells
  • Gram-Postive Bacterial Cells
  • Fungus Cells

Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)

  • Animal Tissue

Genomic DNA Isolation Reagent Kit (Blood/Cultured Cell/Tissue)

Required Materials
  • Microcentrifuge tubes
  • Isopropanol
  • RNase A (10 mg/ml)
  • TE Buffer
  • For the tissue sample: Proteinase K(10 mg/ml), Micropestle

Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

Low yield of DNAIncomplete lysed sampleUse the appropriate method for the lysate preparation based on the amount of the starting materials.
Be sure to add Proteinase K during lysis.
Increase the digestion time or amount of Proteinase K used for lysis.
For tissues, cut the tissue into smaller pieces and ensure the tissue is completely immersed in the Lysis step to obtain optimal lysis.
DNA degradeSample not freshAvoid repeated freeze / thaw cycles of the sample.
Use a new sample for the DNA isolation. Perform the extraction of the fresh material when possible.The yield and quality of DNA isolated is dependent on the type and age of the starting material.
Inappropriate sample storage conditionsStore mammalian tissues at -80°C and bacteria at -20°C until use. The whole blood can be stored at 4°C for no longer than 1-2 days.
DNase contaminantUse the fresh TAE or TBE electrophoresis buffer
Maintain a sterile work environment to avoid contamination from DNases.
Presence of RNARNA contaminationPerform RNase A digestion step during the Step Lysis.
Inhibition of downstream enzymatic reactionsPresence of ethanol in purified DNABefore the DNA Rehydration step, ensure the ethanol was removed completely
  • During operation, always wear a lab coat, disposable gloves, and protective equipment.
  • Check buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.
  • Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.
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