IRIS11 Prestained Protein Ladder

Catalog numberPMI11-0500

Size500 μl

Range3 to 260 kDa

UseAs molecular weight standards for SDS-PAGE electrophoresis

Sample25 μl ($7.2)

$108 Inquire

Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / 9 bands / SDS-polyacrylamide gel electrophoresis

Description

The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Properties

Appearance: Liquid
Color: Blue
Odor: Odorless
Weak pH-value at 25 °C: ~7.5
Boiling point: ~100 °C

Reaction Setup

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Guide for Molecular Weight Estimation (kDa)

Refer to the IRIS11 Prestained Protein Ladder patterns in various electrophoresis conditions:

IRIS11 Prestained Protein Ladder

Important notes: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buﬀer

0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control

5 μl of IRIS11 Prestained Protein Ladder (PMI11) resolves 11 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage Conditions

Stable for up to 2 weeks at 25 °C.
Stable for up to 3 months at 4 °C.
Stable for up to 24 months at -20 °C.

Required Materials but Not Provided

Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge