IRIS11 Prestained Protein Ladder

Catalog numberPMI11-0500

Size500 μl

Range3 to 260 kDa

UseAs molecular weight standards for SDS-PAGE electrophoresis

Sample25 μl ($7.2)

$108 Inquire

Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / 9 bands / SDS-polyacrylamide gel electrophoresis

Description

The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Properties

Appearance: Liquid
Color: Blue
Odor: Odorless
Weak pH-value at 25 °C: ~7.5
Boiling point: ~100 °C

Reaction Setup

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Guide for Molecular Weight Estimation (kDa)

Refer to the IRIS11 Prestained Protein Ladder patterns in various electrophoresis conditions:

IRIS11 Prestained Protein Ladder

Important notes: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buﬀer

0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control

5 μl of IRIS11 Prestained Protein Ladder (PMI11) resolves 11 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage Conditions

Stable for up to 2 weeks at 25 °C.
Stable for up to 3 months at 4 °C.
Stable for up to 24 months at -20 °C.

Required Materials but Not Provided

Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies