BH 1Kb plus DNA Ladder RTU

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-10,000 base pairs. The 3K and 1K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source

PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Reaction Setup

5 μl / well, containing bromophenol blue & xylene cyanol FF as tracking dyes.

Agarose gel electrophoresis of DNA ladders. This is a storage test for the DNA ladder samples of different lot. numbers which were stored at -20 °C specifically since 2016, 2017, 2018, 2019 and 2021 till the test date. As the photo presented, the oldest and latest produced DNA ladder are still functioning the same despite the long storage period. The photo proves the shelf life of this product is as the protocol claimed.

• Stable for up to 6 months at 25 °C.
• Stable for up to 12 months at 4 °C.
• Stable for up to 24 months at -20 °C.

• Horizontal Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge