Total RNA Isolation Kit (Blood/Cultured Cell/Fungus)

The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for the isolation of total RNA from the whole blood, mammalian cells, and bacterial cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with the REL Buffer without phenol extraction or alcohol precipitation. The RNA purified with the Total RNA Isolation Kit is suitable for a variety of routine applications, including the RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA selection. The entire procedure can be completed within 25-40 minutes.

• Sample:

Up to 300 μl of whole bloodUp to 10⁷ mammalian cells

Up to 10⁹ bacterial cellsUp to 10⁸ fungus cells

• Format: Reagent and spin column
• Yield: Up to 30 μg
• Operation time: 25-40 minutes
• Elution volume: 50-200 ul

Kit Contents

• Delivers high-quality total RNA with the fast procedure.
• Ready-to-use RNA for high performance in any downstream application.
• Consistent RNA yield from the starting material with a small amount.

• Fresh Blood/Cultured Mammalian Cells
• Bacterial Cells/Fungus Cells

Refer to the table below to troubleshoot problems that you may encounter when purifying total RNA.

• During the operation, always wear the latex or vinyl gloves while handling reagents and RNA samples to prevent the RNase contamination.
• Add 60 ml of the ethanol (96~100%) to the Buffer W2, and shake before use (see bottle label for volume).
• Check Buffers before use for salt precipitation. Re-dissolve any precipitate by warming up to 37°C.
• The Buffers RA and W1 contain irritants., so please wear gloves when handling these buffers.
• Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.