Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)

Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)
Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)
Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)

Catalog numberPDC09-0100

CategoriesColumn Based

Size100 Reactions

Sample4 rxns ($14.4)

$237.6 Inquire

Note: PureDireX / Purification Kits / Extraction Kit / Blood / Cultured Cell / Fungus


The Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) is designed specifically for genomic DNA isolation from the whole blood, frozen blood, buffy coat, cultured animal/bacterial cells, and fungus. This unique buffer system ensures genomic DNA with high yield and good quality from samples. The spin column is designed to purify or concentrate genomic DNA products which have been previously isolated using buffers. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified genomic DNA is suitable for use in PCR or other enzymatic reactions.

  • Sample
  • Up to 300 µl of the whole bloodCultured animal cells (up to 1 x 107)

    Up to 200 µl of the frozen bloodCultured bacterial cells (up to 1 x 109)

    Up to 200 µl of the buffy coatFungus cells (up to 5 x 107)

  • Format: Reagent and spin column
  • Yield: Up to 50 μg
  • Operation time: Within 60 minutes
  • Elution volume: 50-200 μl

Kit Contents

ContentsPDC09-0100PDC09-0100S (Sample)
Buffer CR100 ml4 ml
Buffer CC35 ml1.5 ml
Buffer CB45 ml2 ml
Buffer W145 ml2 ml
Buffer W2 (Add ethanol)15 ml (60 ml)300 μl × 2 (1.5 ml × 2)
Buffer E10 ml1 ml
Column CC100 pcs4 pcs
Collection Tubes100 pcs4 pcs
  • Delivering high-quality genomic DNA with the fast procedure.
  • Ready-to-use genomic DNA for high performance in any downstream application.
  • Highly purified and high yield genomic DNA can be extracted from various samples.
  • Optimized lysis buffer for the efficient lysis.
  • Designed to rapidly purify high-quality DNA using spin column format.
  • Fresh whole Blood or Buffy Coat
  • Cultured Mammalian Cells
  • Gram-Negative Bacterial Cells
  • Gram-Positive Bacterial Cells
  • Fungus Cells

Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)


Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

Low yield of DNAIncomplete lysed sampleUse the appropriate method for the lysate preparation based on the amount of the starting materials.
Increase the digestion time.
DNA degradeSample not freshAvoid repeated freeze / thaw cycles of the sample.
Use a new sample for the DNA isolation. Perform the extraction of the fresh material when possible.
Inappropriate sample storage conditionsStore culture cell / fungus at -20°C until use. The whole blood can be stored at 4°C for no longer than 1-2 days.
DNase contaminantUse the fresh TAE or TBE electrophoresis buffer.
Maintain a sterile work environment to avoid contamination from DNases.
Presence of RNARNA contaminationPerform RNase A digestion step during Step Lysis.
Inhibition of downstream enzymatic reactionsPresence of ethanol in purified DNADiscard the ethanol of the Buffer W2 flow through from the collection tube. Place the spin cartridge into the collection tube and centrifuge the spin cartridge at maximum speed for 2–3 minutes to completely dry the cartridge.
  • During operation, always wear a lab coat, disposable gloves, and protective equipment.
  • Check buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.
  • Add 60 ml of the ethanol (96–100%) to the Buffer W2, and shake before use.
  • Buffers W1 contain irritants. Wear gloves when handling these buffers.
  • Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.
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