Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)

The Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) is designed specifically for genomic DNA isolation from the whole blood, frozen blood, buffy coat, cultured animal/bacterial cells, and fungus. This unique buffer system ensures genomic DNA with high yield and good quality from samples. The spin column is designed to purify or concentrate genomic DNA products which have been previously isolated using buffers. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified genomic DNA is suitable for use in PCR or other enzymatic reactions.

• Sample

Up to 300 µl of the whole bloodCultured animal cells (up to 1 x 10⁷)

Up to 200 µl of the frozen bloodCultured bacterial cells (up to 1 x 10⁹)

Up to 200 µl of the buffy coatFungus cells (up to 5 x 10⁷)

• Format: Reagent and spin column
• Yield: Up to 50 μg
• Operation time: Within 60 minutes
• Elution volume: 50-200 μl

Kit Contents

• Delivering high-quality genomic DNA with the fast procedure.
• Ready-to-use genomic DNA for high performance in any downstream application.
• Highly purified and high yield genomic DNA can be extracted from various samples.
• Optimized lysis buffer for the efficient lysis.
• Designed to rapidly purify high-quality DNA using spin column format.

• Fresh whole Blood or Buffy Coat
• Cultured Mammalian Cells
• Gram-Negative Bacterial Cells
• Gram-Positive Bacterial Cells
• Fungus Cells

Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

• During operation, always wear a lab coat, disposable gloves, and protective equipment.
• Check buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.
• Add 60 ml of the ethanol (96–100%) to the Buffer W2, and shake before use.
• Buffers W1 contain irritants. Wear gloves when handling these buffers.
• Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.