Improved Method for Measuring Intracellular Ca++ With fluo-3

G T Rijkers, L B Justement, A W Griffioen, J C Cambier

Cytometry. 1990;11(8):923-7.

PMID: 2272255

Abstract:

The accuracy of flow cytometric measurement of intracellular calcium with fluo-3 is compromised by variation in basal fluorescence intensity due to heterogeneity in dye uptake or compartmentalization. We have loaded cells simultaneously with fluo-3 and SNARF-1. When SNARF-1 fluorescence is collected at approximately 600 nm, its intensity does not change upon cell activation. Furthermore, fluo-3 and SNARF-1 fluorescence signals exhibit a linear relationship. The ratio of fluo-3 to SNARF-1 eliminates a significant proportion of variation in fluorescence intensity caused by variation in fluo-3 uptake and thus can be used as a sensitive parameter for measuring changes in [Ca2+]i.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP123632393	Fluo-3		123632-39-3	Price