Production of L-2,3-butanediol by a New Pathway Constructed in Escherichia Coli

S Ui, Y Takusagawa, T Sato, T Ohtsuki, A Mimura, M Ohkuma, T Kudo

Lett Appl Microbiol. 2004;39(6):533-7.

PMID: 15548307

Abstract:

Aims:




A metabolic pathway for L-2,3-butanediol (BD) as the main product has not yet been found. To rectify this situation, we attempted to produce L-BD from diacetyl (DA) by producing simultaneous expression of diacetyl reductase (DAR) and L-2,3-butanediol dehydrogenase (BDH) using transgenic bacteria, Escherichia coli JM109/pBUD-comb.













Methods and results:




The meso-BDH of Klebsiella pneumoniae was used for its DAR activity to convert DA to L-acetoin (AC) and the L-BDH of Brevibacterium saccharolyticum was used to reduce L-AC to L-BD. The respective gene coding each enzyme was connected in tandem to the MCS of pFLAG-CTC (pBUD-comb). The divided addition of DA as a source, addition of 2% glucose, and the combination of static and shaking culture was effective for the production.













Conclusions:




L-BD (2200 mg l(-1)) was generated from 3000 mg l(-1) added of DA, which corresponded to a 73% conversion rate. Meso-BD as a by-product was mixed by 2% at most.













Significance and impact of the study:




An enzyme system for converting DA to L-BD was constructed with a view to using DA-producing bacteria in the future.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP19132060	L-(+)-2,3-Butanediol		19132-06-0	Price