The Diagnosis of Gaucher's Disease in Liver Using 4-methylumbelliferyl-beta-D-glucopyranoside

D M Broadhead, J Butterworth

Clin Chim Acta. 1977 Feb 15;75(1):155-61.

PMID: 14796

Abstract:

1. Cases of Gaucher's disease could not be distinguished from controls by the assay of beta-glucosidase activity in water homogenates of liver using 4-methylumbelliferyl-beta-D glucopyranoside. 2. Two peaks of beta-glucosidase activity were separated by Sephadex G-150 gel filtration in control and Gaucher livers. In the presence of the elution buffer pH profiles of peak I showed a deficiency at pH 3.5-4.5 in Gaucher's disease. Gaucher and control peak II had similar pH profiles with little or no activity at pH 3.0-4.0. 3. A clear distinction between homogenates of Gaucher and control liver was obtained by assay at pH 4.0 in the presence of elution buffer, or of sodium chloride, a component of the elution buffer.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18997574	4-Methylumbelliferyl β-D-glucopyranoside		18997-57-4	Price