Capillary Electrophoresis Analysis of Prostate-Specific Antigen (PSA)

Noemi Farina-Gomez, Diana Navarro-Calderon, Angel Puerta, Monica Gonzalez, José Carlos Diez-Masa, Mercedes de Frutos

Methods Mol Biol. 2019;1972:221-234.

PMID: 30847795

Abstract:

The Capillary Electrophoresis (CE) profile of isoforms (peaks) of a glycoprotein can be useful to show alterations in its posttranslational modifications (PTMs) linked to diseases. These changes can modify the electrophoretic mobility of these isoforms in a minor extent and, therefore, very reproducible CE methods are needed to detect them. In this chapter, a method for the analysis of prostate-specific antigen (PSA) by Capillary Zone Electrophoresis (CZE) with UV detection is detailed. High reproducibility in the separation of a large number of PSA isoforms is achieved by performing capillary conditioning in acid media and by using a background electrolyte (BGE) at pH 8.0 formulated with decamethonium bromide and urea.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP541220	Decamethonium bromide		541-22-0	Price