Activation of Cellulose Membranes With 1,1'-carbonyldiimidazole or 1-cyano-4-dimethylaminopyridinium Tetrafluoroborate as a Basis for the Development of Immunosensors

Daniela Stöllner, Frieder W Scheller, Axel Warsinke

Anal Biochem. 2002 May 15;304(2):157-65.

PMID: 12009691

Abstract:

Methods for the activation of a cellulose dialysis membrane for immunosensor applications have been developed. For activation two reagents, 1,1'-carbonyldiimidazole (CDI) and 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP), were compared with respect to the coupling efficiency for glucose oxidase (GOx) and 1,8-diamino-2,6-dioxaoctane. The maximum level of activation was 2.4 micromol cm(-2) for CDI and 0.2 micromol cm(-2) for CDAP activation. We observed 1.5 microg cm(-2) and 0.4 x 10(-4) U cm(-2) GOx with CDI-activated membranes whereas 1.7 microg cm(-2) and 7.2 x 10(-4) U cm(-2) GOx were observed with CDAP-activated membranes. With 1,8-diamino-2,6-dioxaoctane amino group densities of 0.165 and 0.09 micromol cm(-2) were observed via CDI and CDAP activation, respectively. An amino-modified membrane was used for coupling a ligand (pentapeptide) and an immunoenzymometric assay for hemoglobin A1c was carried out.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP59016567	1-Cyano-4-dimethylaminopyridinium tetrafluoroborate		59016-56-7	Price