Alkaline and Halophilic Protease Production by Bacillus luteus H11 and Its Potential Industrial Applications

Agnieszka Kalwasińska, Urszula Jankiewicz, Tamás Felföldi, Aleksandra Burkowska-But, Maria Swiontek Brzezinska

Food Technol Biotechnol. 2018 Dec;56(4):553-561.

PMID: 30923452

Abstract:

This paper presents the results of the study on the production of protease by Bacillus luteus H11 isolated from an alkaline soda lime. B. luteus H11 was identified as an alkalohalophilic bacterium, and its extracellular serine endoprotease also showed an extreme alkali- and halotolerance. It was remarkably stable in the presence of NaCl up to 5 M. The enzyme was active in a broad range of pH values and temperatures, with an optimum pH of 10.5 and a temperature of 45 °C. It had a molecular mass of about 37 kDa and showed activity against azocasein and a synthetic substrate for the subtilisin-like protease, N-succinyl-l-phenylalanine-p-nitroanilide. The halo-alkaline protease produced by B. luteus H11 seems to be significant from an industrial perspective because of its tolerance towards high salinity and alkalinity as well as its stability against some organic solvents, surfactants and oxidants. These properties make the protease suitable for applications in food, detergent and pharmaceutical industries, and also in environmental bioremediation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2440622	N-Succinyl-L-phenylalanine-p-nitroanilide		2440-62-2	Price