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A Novel Immunoregulatory Protein in Human Colostrum, syntenin-1, for Promoting the Development of IgA-producing Cells From Cord Blood B Cells

A Novel Immunoregulatory Protein in Human Colostrum, syntenin-1, for Promoting the Development of IgA-producing Cells From Cord Blood B Cells

Mostafa M Sira, Taketoshi Yoshida, Makoto Takeuchi, Yoshinori Kashiwayama, Takeshi Futatani, Hirokazu Kanegane, Akiko Sasahara, Yasunori Ito, Mineyuki Mizuguchi, Tsuneo Imanaka, Toshio Miyawaki

Int Immunol. 2009 Sep;21(9):1013-23.

PMID: 19592421

Abstract:

Human colostrum contains many bioactive factors that must promote the development of intestinal mucosal immunity in infants. Especially, the presence of certain cytokines such as transforming growth factor (TGF)-beta or IL-10 has been of great interest for IgA production as a function of mucosal immune response. In the present study, we attempted to investigate whether unidentified factors inducing generation of IgA-producing cells from naive B cells might exist in colostrum. For this purpose, colostrum samples were directly added to a culture consisting of naive B cells and dendritic cells from cord blood and CD40 ligand-transfected L cells, comparing with recombinant IL-10 (rIL-10) and/or rTGF-beta. It was noted that most colostrum samples alone were able to induce IgA-secreting cells at higher levels than rIL-10 and/or rTGF-beta. IgA-inducing activity of colostrum was abolished by neither anti-neutralizing mAbs against IL-10 nor TGF-beta, though partially by anti-IL-6 mAb. We prepared partially purified fractions from both pooled colostrums with and without IgA-inducing activity and comparatively performed quantitative proteomic analysis by two-dimensional difference gel electrophoresis followed by liquid chromatography-mass spectrometry. As a result, syntenin-1 was identified as a candidate for IgA-inducing protein in colostrum. Western blot analysis indicated that levels of syntenin-1 in colostrum samples were correlated with their IgA-inducing activities. Moreover, we demonstrated that recombinant syntenin-1 could induce preferentially IgA production from naive B cells. These results suggest that syntenin-1 serves as one of IgA-inducing factors for B cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411686	IgA from human colostrum			Price

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