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Alginate Dialdehyde Meets Nylon Membrane: A Versatile Platform for Facile and Green Fabrication of Membrane Adsorbers

Alginate Dialdehyde Meets Nylon Membrane: A Versatile Platform for Facile and Green Fabrication of Membrane Adsorbers

M Kamran Khan, Jianquan Luo, Zhaoshuai Wang, Rashid Khan, Xiangrong Chen, Yinhua Wan

J Mater Chem B. 2018 Mar 21;6(11):1640-1649.

PMID: 32254280

Abstract:

Alginate dialdehyde (ADA), a biocompatible polymer, was used as an intermediate layer on a nylon membrane to readily fabricate cation exchange (CEX), metal-affinity (Me-affinity), histidine-affinity (His-affinity) and peptide-affinity (Pep-affinity) membrane adsorbers without any organic solvent usage. All the membrane adsorbers exhibited a high selectivity in the fractionation of a IgG (immunoglobulin)/HSA (human serum albumin) mixture. Along with a high purity of 100%, a high IgG binding capacity of 30, 24, 21 and 28 mg mL-1 (membrane volume) was achieved by the CEX, Me-affinity, His-affinity and Pep-affinity membrane adsorbers in flow-through mode respectively, which is superior to those of the reported membrane adsorbers. Furthermore, the CEX and Pep-affinity adsorbers were tested for the separation of IgG from human plasma solution. The carboxylic groups along with the peptides on the Pep-affinity adsorber captured IgG synergistically with a higher recovery and purity (99% and 98.6%). This synergetic adsorber showed a qmax and dynamic binding capacity (DBC) of 138.4 and 38.7 mg mL-1 respectively for IgG binding which was somewhat higher than that of the reported protein A agarose (having qmax and DBC of 84 and 24.4 mg g-1 respectively). The present work indicated that the ADA layer not only activated the membrane surface to attach various adsorptive ligands under mild conditions, but also reduced non-specific adsorption. Due to the versatile linking function and green reaction conditions, the ADA coating on the nylon membrane is promising for the preparation of diverse membrane adsorbers.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411022	Protein A-Agarose			Price

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