Home
Resources
Publications
Assaying Cell Cycle Status Using Flow Cytometry

Assaying Cell Cycle Status Using Flow Cytometry

Kang Ho Kim, Joel M Sederstrom

Curr Protoc Mol Biol. 2015 Jul 1;111:28.6.1-28.6.11.

PMID: 26131851

Abstract:

In this unit, two protocols are described for analyzing cell cycle status using flow cytometry. The first is based on the simultaneous analysis of proliferation-specific marker (Ki-67) and cellular DNA content, which discriminate resting/quiescent cell populations (G0 cell) and quantify cell cycle distribution (G1, S, or G2/M), respectively. The second is based on differential staining of DNA and RNA through co-staining of Hoechst 33342 and Pyronin Y, which is also useful to identify G0 cells from G1 cells. Along with these methods for analyzing cell cycle status, two additional methods for cell proliferation assays with recent updates of newly developed fluorophores, which allow multiplex analysis of cell cycle status, cell proliferation, and a gene of interest using flow cytometry, are outlined.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP92320	Pyronin Y		92-32-0	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: