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Carbohydrate-independent Antibiofilm Effect of Bothrops Jararacussu Lectin BJcuL on Staphylococcus Aureus

Ananda Pereira Aguilar, Thiago Souza Onofre, Mary Hellen Fabres-Klein, Raphael Contelli Klein, Renato Neves Feio, Tiago Antônio de Oliveira Mendes, Andrea de Oliveira Barros Ribon

Microb Pathog. 2019 Dec;137:103745.

PMID: 31520734

Abstract:

The antivirulence approach to fighting biofilm-based infections caused by Staphylococcus aureus is a promising therapy that has been studied extensively. Here, we compare the antibiofilm activity of a purified lectin from Bothrops jararacussu venom (BJcuL) and commercial lectins obtained from Triticum vulgaris (Wheat Germ Agglutinin, WGA), Bandeiraea simplicifolia BS-II, and Maclura pomifera. Only WGA had antibiofilm activity, although no effect was seen on pre-formed biofilms. The pre-incubation of WGA and BJcuL with their preferential sugars inhibited the biological activity of WGA, but not that of BJcuL, suggesting that biofilm disruption does not involve carbohydrate-recognition domains (CRDs). Quantitative real-time PCR showed that BJcuL promotes modulation of expression of S. aureus genes involved in biofilm formation. Light microscopy revealed cocci and small cell clusters after biofilm formation in the presence of BJcuL, showing that the lectin treatment was unable to completely disrupt biofilm structure. Exposing the free cells to 50 times the minimum inhibitory concentration of gentamicin or ciprofloxacin did not prevent biofilm reestablishment, although inhibition was stronger than in the control (no lectin). This disruption of the biofilm architecture can expose the bacterial cell and may facilitate clearance by the immune system.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR424657 Lectin from Triticum vulgaris (wheat) Lectin from Triticum vulgaris (wheat) Price
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