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[Cloning of Streptavidin Gene From Streptomyces Avidinii and Its Expression in Escherichia Coli. Secretion of Streptavidin by E. Coli Cells]

[Cloning of Streptavidin Gene From Streptomyces Avidinii and Its Expression in Escherichia Coli. Secretion of Streptavidin by E. Coli Cells]

V P Veĭko, L B Gul'ko, N A Okorokova, N A D'iakov, V G Debabov

Bioorg Khim. 1999 Mar;25(3):184-8.

PMID: 10382041

Abstract:

The streptavidin gene from Streptomyces avidinii was cloned, an expression plasmid constructed, and a highly effective strain producer of streptavidin created. It was shown that the leader peptide of streptavidin ensures the effective secretion of this protein into the periplasmic space of Escherichia coli cells. The degradation site of the leader peptide was detected. Upon treatment with the total fraction of proteases secreted by S. avidinii into the culture medium, "core" streptavidin was obtained, which retained the biotin-binding function.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9013201	Streptavidin from Streptomyces avidinii		9013-20-1	Price

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