Home
Resources
Publications
Directing Membrane Chromatography to Manufacture α1-antitrypsin From Human Plasma Fraction IV

Directing Membrane Chromatography to Manufacture α1-antitrypsin From Human Plasma Fraction IV

Jinxin Fan, Jianquan Luo, Weijie Song, Xiangrong Chen, Yinhua Wan

J Chromatogr A. 2015 Dec 4;1423:63-70.

PMID: 26518493

Abstract:

The surging demand for plasma proteins, mainly driven by the growing market and the development of new therapeutic indications, is promoting manufacturers to improve the throughput of plasma proteins. Due to the inherent convective mass transfer, membrane chromatography has been proved to be an efficient approach for extracting a small amount of target proteins from large-volume feed. In this study, α1-antitrypsin (AAT) was extracted from human plasma fraction IV by a two-step membrane chromatography. An anion-exchange membrane chromatography (AEMC) was used to capture the plasma proteins in bind/elute mode, and the obtained effluent was further polished by a hydrophobic interaction membrane chromatography (HIMC) in flow-through mode. Under optimal conditions, the recovery and purity of AAT achieved 87.0% and 0.58 AAT/protein (g/g) by AEMC, respectively. After the precise polishing by HIMC, the purity of AAT was 1.22 AAT/protein (g/g). The comparison results showed that membrane chromatography outperformed column chromatography in both steps because of its high throughput. This two-step membrane chromatography could obtain an AAT recovery of 83.3% and an activity recovery of 91.4%. The outcome of this work not only offers an alternative process for protein purification from plasma, but also provides guidelines for manufacturing product from a large-volume feed with multi-components by membrane chromatography.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9041923	α1-Antitrypsin from human plasma		9041-92-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: