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Efficient Transduction of Mature CD83+ Dendritic Cells Using Recombinant Adenovirus Suppressed T Cell Stimulatory Capacity

Efficient Transduction of Mature CD83+ Dendritic Cells Using Recombinant Adenovirus Suppressed T Cell Stimulatory Capacity

H Jonuleit, T Tüting, J Steitz, J Brück, A Giesecke, K Steinbrink, J Knop, A H Enk

Gene Ther. 2000 Feb;7(3):249-54.

PMID: 10694802

Abstract:

We have developed a culture method for the foreign serum-free generation of highly immunostimulatory, CD83+ human dendritic cells (DC). In this study, we evaluated the feasibility and consequences of endogenously expressing antigens in mature DC using adenoviral vectors. Transduction of DC with Ad-EGFP demonstrated endogenous fluorescence in 50-85% of CD83+ DC. Ad-transduced DC stimulated the proliferation of allogeneic CD8+ and CD4+ T cells at low DC: T cell ratios. However, at high DC: T cell ratios the stimulatory capacity of Ad-transduced DC was suppressed. This immunosuppressive effect was confirmed by demonstrating that the stimulatory function of untreated DC could be suppressed in a dose-dependent manner by addition of Ad-transduced DC. Furthermore, transwell experiments suggested that direct cell contact was required. Taken together, our results demonstrate the feasibility of efficiently expressing antigens in CD83+ DC using adenoviruses. However, immunosuppressive effects must be considered and carefully studied before Ad-transduced DC are employed for clinical trials. Gene Therapy (2000) 7, 249-254.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413168	CD83 human			Price

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