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Enhanced Soluble Production of Biologically Active Recombinant Human p38 Mitogen-Activated-Protein Kinase (MAPK) in Escherichia Coli

Sunil K Khattar, Pankaj Gulati, Prabuddha K Kundu, Vibhuti Singh, Usha Bughani, Malini Bajpai, Kulvinder S Saini

Protein Pept Lett. 2007;14(8):756-60.

PMID: 17979814

Abstract:

The conditions were optimized for maximum soluble yield of biologically active recombinant p38alpha mitogen activated protein kinase (MAPK) vis-à-vis insoluble fraction (inclusion body formation). This study reports a rapid, economical and single step purification process for the overproduction of GST tagged p38alpha MAPK. A yield of 18 mg of highly purified and soluble protein per liter of bacterial culture within 6 h timeframe was achieved. The purified protein was found to be biologically suitable for phosphorylation by upstream kinases and was catalytically active. We further demonstrated that our in-house p38alpha MAPK is more potent (>30%) than a commercially available enzyme.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR4248198 p38α, active, GST tagged human p38α, active, GST tagged human Price
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