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Fractionation of Fish Oil Fatty Acid Methyl Esters by Means of Argentation and Reversed-Phase High-Performance Liquid Chromatography, and Its Utility in Total Fatty Acid Analysis

M Ozcimder, W E Hammers

J Chromatogr. 1980 Jan 18;187(2):307-17.

PMID: 7380922

Abstract:

The utility of reversed-phase and argentation high-performance liquid chromatography (HPLC) as pre-fractionation methods in fatty acid analysis is discussed. Both HPLC modes were applied to cod liver oil fatty acid methyl esters. Apart from positional isomers, the fractions obtained by reversed-phase HPLC were analysed by gas-liquid chromatography and appear to be free of the usually occurring "critical pairs". The mechanism of retention of the fatty acid methyl esters on low-loaded silver nitrate-impregnated silicas is discussed. It is shown that argentation HPLC is a rapid semi-preparative pre-fractionation method for highly unsaturated fatty esters with 3-6 double bonds.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR4247484 Cod liver oil fatty acid methyl esters Cod liver oil fatty acid methyl esters Price
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