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Gas Chromatography-Vacuum Ultraviolet Spectroscopy for Analysis of Fatty Acid Methyl Esters

Gas Chromatography-Vacuum Ultraviolet Spectroscopy for Analysis of Fatty Acid Methyl Esters

Hui Fan, Jonathan Smuts, Ling Bai, Phillip Walsh, Daniel W Armstrong, Kevin A Schug

Food Chem. 2016 Mar 1;194:265-71.

PMID: 26471553

Abstract:

A new vacuum ultraviolet (VUV) detector for gas chromatography was recently developed and applied to fatty acid methyl ester (FAME) analysis. VUV detection features full spectral acquisition in a wavelength range of 115-240nm, where virtually all chemical species absorb. VUV absorption spectra of 37 FAMEs, including saturated, monounsaturated, and polyunsaturated types were recorded. Unsaturated FAMEs show significantly different gas phase absorption profiles than saturated ones, and these classes can be easily distinguished with the VUV detector. Another advantage includes differentiating cis/trans-isomeric FAMEs (e.g. oleic acid methyl ester and linoleic acid methyl ester isomers) and the ability to use VUV data analysis software for deconvolution of co-eluting signals. As a universal detector, VUV also provides high specificity, sensitivity, and a fast data acquisition rate, making it a powerful tool for fatty acid screening when combined with gas chromatography. The fatty acid profile of several food oil samples (olive, canola, vegetable, corn, sunflower and peanut oils) were analyzed in this study to demonstrate applicability to real world samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4247301	Linoleic acid methyl ester, cis/trans-isomers			Price

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