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Immunostaining for Rapid Diagnosis of Acute Promyelocytic Leukemia With the tetramethylrhodamine-5-isothiocyanate-conjugated Anti-Promyelocytic Leukemia Monoclonal Antibody PG-M3

Immunostaining for Rapid Diagnosis of Acute Promyelocytic Leukemia With the tetramethylrhodamine-5-isothiocyanate-conjugated Anti-Promyelocytic Leukemia Monoclonal Antibody PG-M3

Khaled M Alayed, L Jeffrey Medeiros, Danh Phan, Chinemerem Ojiaku, Jyoti Patel, Jan Paul V Yap, Yen McCord, Janaya S Woods, Sergej Konoplev, Carlos E Bueso-Ramos, Steven R Reyes

Arch Pathol Lab Med. 2013 Nov;137(11):1669-73.

PMID: 24168508

Abstract:

Context:
Anti-promyelocytic leukemia (PML) immunofluorescence staining is a known diagnostic tool for rapid diagnosis of acute promyelocytic leukemia (APL).
Objective:
We describe our methods using the recently developed, commercially available, tetramethylrhodamine-5-isothiocyanate-labeled PG-M3 anti-PML antibody for APL testing.
Design:
Immunofluorescence staining with the tetramethylrhodamine-5-isothiocyanate-labeled PG-M3 antibody was used to detect PML-RARA in bone marrow aspirate and/or peripheral blood smears from 30 patients with acute leukemia. The results were compared with those of concurrent testing with our in-house polyclonal anti-PML antibody and with established tests.
Results:
All APL cases showed a positive (fine/microgranular) immunofluorescence staining pattern, whereas non-APL cases showed a negative (chunky/macrogranular) pattern. These results, which were available within 2 hours, were validated by testing with the polyclonal anti-PML antibody and with established cytogenetic and molecular testing methods.
Conclusions:
We validated the utility of the tetramethylrhodamine-5-isothiocyanate-labeled anti-PML antibody PG-M3 for the diagnosis of APL. Our results indicate that immunofluorescence staining with this antibody is a rapid and reliable method for the diagnosis of APL.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP80724192	Tetramethylrhodamine-5-isothiocyanate		80724-19-2	Price

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