Home
Resources
Publications
Isolation of endopeptidase-24.11 (EC 3.4.24.11, "Enkephalinase") From the Pig Stomach. Hydrolysis of Substance P, Gastrin-Releasing Peptide 10, [Leu5] Enkephalin, and [Met5] Enkephalin

Isolation of endopeptidase-24.11 (EC 3.4.24.11, "Enkephalinase") From the Pig Stomach. Hydrolysis of Substance P, Gastrin-Releasing Peptide 10, [Leu5] Enkephalin, and [Met5] Enkephalin

N W Bunnett, A J Turner, J Hryszko, R Kobayashi, J H Walsh

Gastroenterology. 1988 Oct;95(4):952-7.

PMID: 2457534

Abstract:

The purpose of this investigation was to isolate the cell-surface enzyme endopeptidase-24.11 from the stomach wall of the pig and to examine the hydrolysis of the gastric neuropeptides. Endopeptidase-24.11 was isolated from gastric membranes by immunoadsorbent chromatography using a monoclonal antibody to porcine kidney endopeptidase-24.11. The enzyme was purified with a yield of 1.2 micrograms/g wet wt of fundic muscle. A single polypeptide chain of apparent subunit molecular weight of 90,000 was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Gastric endopeptidase-24.11 hydrolyzed substance P, gastrin-releasing peptide 10, [Leu5] enkephalin, and [Met5] enkephalin by cleavage of peptide bonds on the N-terminal side of hydrophobic amino acids. The enzymatic activity was inhibited completely by phosphoramidon (10(-6) M) and strongly by 1,10-phenanthroline (10(-3) M), but was unaffected by captopril (10(-5) M).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42412318	Endopeptidase, Neutral, Porcine Kidney			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: