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Kinetics of the Endocytotic Pathway of Low Density Lipoprotein (LDL) in Human Endothelial Cells Line Under Shear Stress: An in Vitro Confocal Microscopy Study

Kinetics of the Endocytotic Pathway of Low Density Lipoprotein (LDL) in Human Endothelial Cells Line Under Shear Stress: An in Vitro Confocal Microscopy Study

M Traoré, R J Sun, S Fawzi-Grancher, D Dumas, X Qing, R Santus, J-F Stoltz, S Muller

Clin Hemorheol Microcirc. 2005;33(3):243-51.

PMID: 16215290

Abstract:

We studied the effect of mechanical forces (shear stress) on the kinetics of internalization of native LDL and ox-LDL in endothelial cell line ECV304. This study was performed by using Confocal microscopy and FRET with two carbocyanine dyes, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiO) as the donor and 3,3'-dioctadecyloxacarbocyanine perchlorate (DiI) as the receptor. The cells were incubated with a culture medium containing either 10 microg/ml DiI-LDL or DiO-LDL in static conditions or subjected to a laminar flow under a Confocal Laser Scanning Microscope (SP2 Leica, Germany). The results showed: (1) the possibility to evaluate the kinetics of LDL endocytosis in living cells, (2) shear stress in comparison with control group more effectively enhanced LDL uptake, (3) ox-LDL (>50 microg/ml) >4 hours incubation was found to affect the cells as reflected by their detachment at low shear stress.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP34215571	3,3′-Dioctadecyloxacarbocyanine perchlorate		34215-57-1	Price

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