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Making Weak Antigens Strong: Cross-Linking Peptides to KLH With Maleimide

Making Weak Antigens Strong: Cross-Linking Peptides to KLH With Maleimide

Edward A Greenfield, James DeCaprio, Mohan Brahmandam

Cold Spring Harb Protoc. 2018 Oct 1;2018(10).

PMID: 30275080

Abstract:

Haptens, which are small antigens such as peptides and drug compounds, are very weakly or nonimmunogenic by themselves and require the assistance of carrier proteins: complex molecules capable of eliciting a strong immune response in the host on injection. The haptens serve as epitopes for binding to the antibodies on the B-cell surface, and the carriers provide the MHC class II-T-cell receptor binding sites. Keyhole limpet hemocyanin (KLH) is one of the most widely used of such carrier proteins. KLH-hapten conjugates are commonly used in antibody generation in a variety of hosts such as mice, rats, and rabbits. Because KLH is harvested from mollusks, it is physiologically distant from mammalian species and less likely to produce antibodies that cross-react mammalian antigens. Maleimide activation of carrier proteins makes it possible to conjugate sulfhydryl-containing haptens, and hence this chemistry is widely used for conjugating KLH with haptens.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411632	Maleimide Activated KLH			Price

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