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Monoclonal Antibody FDC-6 Exhibits Binding to Human Plasma Fibronectin: A Caveat for Cervicovaginal Oncofetal Fibronectin Testing?

Monoclonal Antibody FDC-6 Exhibits Binding to Human Plasma Fibronectin: A Caveat for Cervicovaginal Oncofetal Fibronectin Testing?

R F Feinberg, C L Wang

Am J Obstet Gynecol. 1994 Nov;171(5):1302-8.

PMID: 7977538

Abstract:

Objective:
Monoclonal antibody FDC-6 has been used clinically to measure cervicovaginal fibronectin and to predict patients at risk for preterm labor. Because bleeding is often a complicating factor during pregnancy, the aim of this study was to determine whether FDC-6 reactive fibronectin is present in normal human plasma.
Study design:
Quantitative Western immunoblots and a commercially available enzyme-linked immunosorbent assay were used to measure FDC-6 binding to nonpregnant human and bovine plasma fibronectin. FDC-6 binding to whole sera was also assessed, as was the effect of enzymatic deglycosylation of human plasma fibronectin.
Results:
Both assays demonstrated measurable FDC-6-reactive fibronectin in nonpregnant human blood, with plasma concentrations ranging from 3 to 12 micrograms/ml (1% to 4% of total plasma fibronectin). FDC-6 had no detectable binding activity to bovine plasma fibronectin. De-O-glycosylated human plasma fibronectin had markedly reduced binding activity for FDC-6.
Conclusions:
FDC-6 binds specifically to a clinically significant percentage of circulating O-glycosylated human plasma fibronectin isoforms that are not associated with pregnancy. This caveat should be considered when pregnant patients are evaluated for the presence of FDC-6 reactive oncofetal fibronectin in the cervix or vagina.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4241157	Fibronectin bovine plasma			Price

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