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N-chlorotaurine, a Novel Endogenous Antimicrobial Agent: Tolerability Testing in a Mouse Model

N-chlorotaurine, a Novel Endogenous Antimicrobial Agent: Tolerability Testing in a Mouse Model

A Neher, M Nagl, A Schrott-Fischer, H Ichiki, W Gottardi, A R Gunkel, K Stephan

Arch Otolaryngol Head Neck Surg. 2001 May;127(5):530-3.

PMID: 11346428

Abstract:

Objective:
To investigate the tolerability of N-chlorotaurine, a new antimicrobial agent, by application to the middle ear in a mouse model.
Methods:
Five BALB/c mice were each injected through the tympanic membrane with 5 microL of 0.1%, 1.0%, and 10% N-chlorotaurine and compared with animals in which 0.9% isotonic sodium chloride solution, 0.2% gentamicin sulfate, and 0.25% trimethyltin chloride were instilled. Auditory brainstem responses to clicks were evaluated repeatedly between 4 and 75 days after injection, and histologic investigations of the inner ear were performed subsequently. Three additional groups of mice were injected with isotonic sodium chloride solution, 1.0% N-chlorotaurine, and 0.25% trimethyltin, and brainstem responses to tone bursts of 8, 16, and 32 kHz were tested. In addition, the middle ear was examined histologically.
Results:
Mice treated with isotonic sodium chloride solution, 0.1% N-chlorotaurine, and 0.2% gentamicin sulfate did not show changes in response threshold. Treatment with 1.0% and 10% N-chlorotaurine caused a reversible increase in auditory brainstem response threshold by 20 dB 4 days after application because of local irritation around the perforation of the tympanic membrane. In contrast, 0.25% trimethyltin showed a permanent elevation of auditory brainstem response threshold of 10 to 15 dB and a scattered loss of outer hair cells predominantly in the apical turn. No alterations of the inner ear were observed in the other treatment groups. The mucous membrane of the middle ear remained unaffected in all test groups.
Conclusion:
Application of N-chlorotaurine to the middle ear is well tolerated without adverse effects and may be a useful new endogenous antimicrobial agent for local treatment of otologic infections.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1066451	Trimethyltin chloride solution		1066-45-1	Price

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