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Production of a Monoclonal Antibody Against Hemorrhagic Activity of Crotalus Atrox (Western Diamondback Rattlesnake) Venom

Production of a Monoclonal Antibody Against Hemorrhagic Activity of Crotalus Atrox (Western Diamondback Rattlesnake) Venom

J C Perez, V E Garcia, S Y Huang

Toxicon. 1984;22(6):967-73.

PMID: 6523516

Abstract:

Crotalid venoms have cytotoxic properties which could be useful in medical research. Crotalus atrox venom-hyperimmunized mouse spleen cells were fused with SP2/0 myeloma cells. Forty-one wells containing the hybridoma cells were positive for C. atrox venom, as determined by the enzyme linked immunosorbent assay (ELISA). Cell line 1-e12 was cloned and used to produce ascites tumors in BALB/c mice. The monoclonal antibody produced by cloned and subcultured 1-e12 cells reacted with both C. atrox venom and six other venoms in the ELISA and neutralized the hemorrhagic activity of crude C. atrox venom. A series of monoclonal antibodies could be used in studying the nature of snake venoms.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4245245	Snake venom from Crotalus atrox (Western Diamondback Rattlesnake)			Price

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