Home
Resources
Publications
Radioimmunoassay of Iodinated Gentamicin

Radioimmunoassay of Iodinated Gentamicin

A Broughton, J E Strong

Clin Chim Acta. 1976 Jan 2;66(1):125-9.

PMID: 944112

Abstract:

A radioimmunoassay is described for the aminoglycoside antibiotic, gentamicin. Iodinated gentamicin was prepared using an 125I-labeled acylating agent: 3-(4-hydroxyphenyl)propionic acid N-hydroxysuccinimide ester which spontaneously reacts with gentamicin to produce an iodinated conjugate. The radioimmunoassay with iodinated gentamicin produced a straight line on a logit/log plot yielding an assay sensitivity of 80 pg. A Scatchard plot plus an equilibrium technique were used to estimate the affinity constant at 8 X 10(9) 1 mol-1 and 10.5 X 10(9) 1 mol-1, respectively. Kanamycin and neomycin, two chemically related aminoglycoside antibiotics, did not crossreact with gentamicin until concentrations were 10(5) greater than those found in blood. Assay of patients' serum samples by both a microbial assay and radioimmunoassay resulted in a statistically significant (p less than 0.001) correlation between the two methods.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP34071959	3-(4-Hydroxyphenyl)propionic acid N-hydroxysuccinimide ester		34071-95-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: