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Simultaneous Enantiospecific Separation and Quantitation of Mephenytoin and Its Metabolites Nirvanol and 4'-hydroxymephenytoin in Human Plasma by Liquid Chromatography

Simultaneous Enantiospecific Separation and Quantitation of Mephenytoin and Its Metabolites Nirvanol and 4'-hydroxymephenytoin in Human Plasma by Liquid Chromatography

Britt Jansson, Ulrika S H Simonsson, Michael Ashton

J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 5;791(1-2):179-91.

PMID: 12798178

Abstract:

A high-performance liquid chromatographic method for the enantiospecific quantitation of S- and R-mephenytoin and its metabolites S- and R-nirvanol and S- and R-4'-hydroxymephenytoin in plasma is described. The compounds were separated using a reversed-phase C(2) column in tandem with a chiral alpha(1)-acid glycoprotein column and were detected using ultraviolet detection at 205 nm. The lower limit of quantification was 10 ng/ml for all compounds using 0.5 ml human plasma (intra-day coefficient of variation <13%, accuracy <+/-20%). The method was validated for human plasma in the concentration range 10-2000 ng/ml for each of the six compounds. The method allows for the simultaneous characterisation of the metabolic capacity of two human drug-metabolising enzymes, CYP2C19 and CYP2B6, and may be used when investigating polymorphisms or changes in activity of these two enzymes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP65567320	(R)-(−)-Nirvanol		65567-32-0	Price

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