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Tentacle-type Immobilized Metal Affinity Cryogel for Invertase Purification From Saccharomyces Cerevisiae

Tentacle-type Immobilized Metal Affinity Cryogel for Invertase Purification From Saccharomyces Cerevisiae

Kemal Çetin, Işık Perçin, Fatma Denizli, Adil Denizli

Artif Cells Nanomed Biotechnol. 2017 Nov;45(7):1431-1439.

PMID: 27776424

Abstract:

The aim of this study is to investigate the usability of cryogel columns for the purification of invertase from Saccharomyces cerevisiae. Poly(2-hydroxyethyl methacrylate) monolithic columns were produced via cryogelation. Ester groups of the poly(2-hydroxyethyl methacrylate) structure were then converted to imine groups by the reaction with poly(ethylene imine) in the presence of NaHCO3. Transition metal ions, Cu(II), Co(II), and Ni(II), were chelated on the PEI-modified cryogel columns. Purification of invertase from natural source namely S. cerevisiae was also studied, and the purification fold values were obtained as 41.350, 44.714, and 30.302 for Cu(II)-chelated, Co(II)-chelated, and Ni(II)-chelated PHEMA/PEI columns, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001574	Invertase from baker's yeast (S. cerevisiae)		9001-57-4	Price

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