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Use of Fluorescein Isothiocyanate Isomer I to Study the Mechanism of Intestinal Absorption of Fucoidan Sulfate in Vivo and in Vitro

Use of Fluorescein Isothiocyanate Isomer I to Study the Mechanism of Intestinal Absorption of Fucoidan Sulfate in Vivo and in Vitro

E Zhang, Fulong Chu, Lixu Xu, Hao Liang, Shuliang Song, Aiguo Ji

Biopharm Drug Dispos. 2018 Jun;39(6):298-307.

PMID: 29904925

Abstract:

A new method to label fucoidan sulfate was established with tyramine and fluorescein isothiocyanate isomer I (FITC). Fluorescence spectrophotometry and high performance liquid chromatography verified the successful labelling of fucoidan by FITC. The results of the single-pass intestinal perfusion indicated that the jejunum and ileum are the main absorption sites, and there was carrier saturation. In addition, fucoidan sulfate at 1 mg/ml had no inhibitory effect on Caco-2 cell proliferation. Studies on the transmembrane transport mechanism showed that fucoidan can be absorbed because the apparent permeability coefficient of the drugs (Papp ) A → B was 3.78 + 0.03 ×10-6 and that of B → A was 1.42 + 0.19 ×10-6 . The peak absorption of fucoidan occurred at 120 min after administration; moreover, the higher the concentration used, the worse the absorption was, suggesting the saturation of transport carriers. The absorption was temperature dependent: the absorption at 37°C was much better than that at 4°C. Further, the absorption of fucoidan sulfate might rely on clathrin endocytosis as chlorpromazine (10 μg/ml) significantly inhibited it.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP3326327	Fluorescein isothiocyanate isomer I		3326-32-7	Price

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