Urodynamic Effects of Intravesical Administration of the New small/intermediate Conductance Calcium Activated Potassium Channel Activator NS309 in Freely Moving, Conscious Rats

Raj Kumar Pandita, Lars Christian B Rønn, Bo Skaaning Jensen, Karl-Erik Andersson

J Urol. 2006 Sep;176(3):1220-4.

PMID: 16890729

Abstract:

Purpose:




We investigated the effects of the new K+ channel activator NS309, which acts on small and intermediate conductance Ca2+ activated K+ channels, on detrusor activity in normal rats.













Materials and methods:




NS309 was given intravesically at different concentrations to conscious female Sprague-Dawley rats undergoing continuous cystometry. The effects of the drug on oxyhemoglobin induced detrusor overactivity were also tested.













Results:




Intravesical NS309 (100, 300 and 1,000 ng ml(-1)) increased bladder capacity, micturition volume and intercontraction intervals in a concentration dependent way. NS309 (1,000 ng ml(-1)) given intravesically for 1 hour before instillation of intravesical oxyhemoglobin (250 microM) decreased or completely prevented the detrusor overactivity induced by oxyhemoglobin.













Conclusions:




Opening small and intermediate conductance Ca2+ activated K+ channels with NS309 given intravesically increased bladder capacity, micturition volume and intercontraction intervals in a concentration dependent way and prevented oxyhemoglobin induced detrusor overactivity. Results suggest that these channels can be interesting targets for drugs aiming to control micturition.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18711165	NS309		18711-16-5	Price