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Mixed-type Inhibition of Bovine Lung Angiotensin Converting Enzyme by Lisinopril and Its Dansyl Derivative

G Uçar, I Ozer

Biochem Pharmacol. 1992 Aug 4;44(3):565-70.

PMID: 1324678

Abstract:

The steady-state inhibition of bovine lung angiotensin converting enzyme (ACE; EC 3.4.15.1) by the slow-binding inhibitor lisinopril and its dansyl derivative conformed to a linear mixed inhibition model with inhibitor binding to ES as well as to E. Studied at pH8, 35 degrees, and using N-(3-[2-furyl]-acryloyl)phe-gly-gly as substrate, the approach to steady-state activity at different substrate concentrations pointed to slow isomerizations in both EI and EIS. While an inhibitory scheme involving a single I-binding site adequately accounts for the data presented, information relating to the primary structure of ACE brings up a two-site alternative which remains to be tested.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP64967391 N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly 64967-39-1 Price
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