CAS 9000-69-5 Pectin - Analytical Products / Alfa Chemistry

CAT	AP9000695
CAS	9000-69-5
MDL Number	MFCD00081838
EC Number	232-553-0

Description	for use with Total Dietary Fiber Control Kit, TDF-C10
Form	lyophilized powder
Size	1G
Storage Conditions	2-8°C

Characterization and antioxidant activity of pectin from Indonesian mangosteen ( Garcinia mangostana L.) rind

Nasrul Wathoni, Chu Yuan Shan, Wong Yi Shan, Tina Rostinawati, Raden Bayu Indradi, Rimadani Pratiwi, Muchtaridi Muchtaridi

Heliyon. 2019 Aug 13;5(8):e02299.

PMID: 31453406

Chemistry and Uses of Pectin--A Review

B R Thakur, R K Singh, A K Handa

Crit Rev Food Sci Nutr. 1997 Feb;37(1):47-73.

PMID: 9067088

Dietary Fiber Pectin Ameliorates Experimental Colitis in a Neutral Sugar Side Chain-Dependent Manner

Keita Ishisono, Toshiyuki Mano, Tomio Yabe, Kohji Kitaguchi

Front Immunol. 2019 Dec 19;10:2979.

PMID: 31921214

Effect of Pectin on the Expression of Proteins Associated With Mitochondrial Biogenesis and Cell Senescence in HT29-Human Colorectal Adenocarcinoma Cells

José Javier Zamorano-León, Sandra Ballesteros, Natalia de Las Heras, Luis Alvarez-Sala, Mariano de la Serna-Soto, Khaoula Zekri-Nechar, Gala Freixer, Bibiana Calvo-Rico, Zhengguang Yang, José Manuel García-García, etc.

Prev Nutr Food Sci. 2019 Jun;24(2):187-196.

PMID: 31328124

Pectin Structure and Biosynthesis

Debra Mohnen

Curr Opin Plant Biol. 2008 Jun;11(3):266-77.

PMID: 18486536

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Case Study

Pectin Used for the Preparation of a Non-Enzymatic Electrochemical Sensor for Creatinine Detection

Hou, Hongming, et al. Microchemical Journal 209 (2025): 112783.

Pectin was utilized as a functional additive to fabricate a non-enzymatic electrochemical sensor for creatinine detection, offering a promising platform for non-invasive monitoring of chronic kidney disease. The sensor, denoted as Pectin-Cu/SPCE, was constructed by electrochemically depositing copper onto a screen-printed carbon electrode (SPCE) in the presence of pectin.
To fabricate the electrode, a precursor solution containing 25 mg/mL CuSO₄·5H₂O and 1.5 mg/mL pectin was drop-cast onto the working surface (radius = 2.5 mm) of a cleaned SPCE. Electrodeposition was performed under a constant potential of -0.6 V for 200 seconds using a CHI650E electrochemical workstation. The coordination between Cu²⁺ and pectin carboxyl groups ensured a uniform deposition of copper. After deposition, the electrode was rinsed with deionized water and vacuum-dried.
For comparison, control electrodes were prepared using Cu²⁺-only and pectin-only solutions. Among them, the Pectin-Cu/SPCE sensor demonstrated the highest sensitivity, achieving a low detection limit of 9.6 µM and a linear detection range of 20-1000 µM for creatinine. This enhanced performance was attributed to improved ion exchange and accelerated electron transfer facilitated by the Cu²⁺-pectin matrix.
This work demonstrates the utility of pectin in electrodeposition processes, particularly for developing sensitive and low-cost biosensors for clinical diagnostics.

Pectin Used for the Preparation of Antibacterial Composite Packaging Films via Ion Exchange Crosslinking

Liu, C., Li, N., Niu, L., Li, X., Feng, J., & Liu, Z. (2025). Carbohydrate Research, 109412.

Pectin, a naturally derived polysaccharide, was employed in the fabrication of biodegradable and antibacterial composite films for potential food packaging applications. In this study, pectin was blended with sodium alginate (SA) to enhance film-forming properties, and glycerol was incorporated as a plasticizer to improve film flexibility.
Using the casting method, aqueous film-forming solutions containing varying ratios of pectin, SA, glycerol, and water were prepared and allowed to stand for 24 hours to eliminate entrapped air. Approximately 10 g of the solution was then cast into molds to form uniform films, including single-component pectin and SA films, as well as pectin/SA blend films.
To introduce antibacterial properties, the freshly prepared SA and pectin/SA films (prior to drying) were immersed in a 5% ZnCl₂ aqueous solution, facilitating an ion exchange reaction. Zinc ions replaced sodium ions in the matrix, resulting in the formation of a semi-interpenetrating network structure (pectin/ZA). The resulting films were thoroughly rinsed with deionized water to remove residual zinc and subsequently air-dried for 24 hours to stabilize the composite structure.
This approach highlights pectin's critical role in the development of bio-based, zinc-reinforced antibacterial films. The ion exchange crosslinking with Zn²⁺ not only imparts antimicrobial activity but also contributes to improved structural integrity, making pectin an essential component in the preparation of eco-friendly packaging materials.

Pectin Used for the Preparation of Stabilized Liposome Delivery Systems via Sequential Protein and Polysaccharide Coating

Ren, Kunyu, et al. Food Chemistry (2025): 143164.

In this study, pectin was employed to prepare a double-layer liposomal delivery system with enhanced stability and controlled release properties. The preparation began with the formation of Lip-7S, a liposome-protein complex. Specifically, an equal volume of 10 mg/mL 7S protein solution was added to the preformed liposome dispersion, resulting in Lip-7S through protein-liposome interactions.
Subsequently, Lip-7S was further coated with varying concentrations of pectin solution (1-5 mg/mL), maintaining a 1:1 volume ratio, to obtain Lip-7S-Pec samples with final pectin concentrations of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%. This step facilitated the formation of a secondary pectin layer on the liposomal surface, effectively modifying surface properties.
The resulting structures were characterized by dynamic light scattering and transmission electron microscopy (TEM), which confirmed an increase in particle size, peaking at 282.96 ± 2.01 nm at 0.4% pectin. Further, the system was evaluated for encapsulation efficiency (60.4% in Lip-7S-Mr-Pec), and in vitro digestion was performed to assess pectin's role in delaying gastric degradation and controlling release of encapsulated Morin.
This sequential assembly method demonstrates pectin's applicability in designing stable, multilayered liposome systems for advanced drug delivery.