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1,2-Diarylethylenediamines as Pre-Column Fluorescence Derivatization Reagents in High-Performance Liquid Chromatographic Determination of Catecholamines in Urine and Plasma

1,2-Diarylethylenediamines as Pre-Column Fluorescence Derivatization Reagents in High-Performance Liquid Chromatographic Determination of Catecholamines in Urine and Plasma

Y Umegae, H Nohta, M Lee, Y Ohkura

Chem Pharm Bull (Tokyo). 1990 Aug;38(8):2293-5.

PMID: 2279295

Abstract:

Meso- and dl-1,2-diarylethylenediamines (14 species) were evaluated for pre-column fluorescence derivatization reagents in the high-performance liquid chromatographic determination of catecholamines (norepinephrine, epinephrine and dopamine) in human urine and plasma. Of the compounds, meso-1,2-bis(4-methoxyphenyl)ethylenediamine was most preferable for all the catecholamines in terms of sensitivity and selectivity. The detection limit for each catecholamine is approximately 0.5 fmol in a 50-microliters injection volume.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP58520459	meso-1,2-Bis(4-methoxyphenyl)ethylenediamine		58520-45-9	Price

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