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1,2-Dioleoylglycerol Method for Pancreatic Lipase Catalytic Activity in Serum

1,2-Dioleoylglycerol Method for Pancreatic Lipase Catalytic Activity in Serum

Yoshiaki Iizuka, Shigeru Ueda, Toshiro Hanada, Wataru Tani, Hiroshi Adachi, Riichi Haga, Mari Yamaguchi, Wataru Kurotani, Mitsuo Sekiguchi, Dongchon Kang, Shin-ichi Sakasegawa

Clin Chem Lab Med. 2011 Nov 30;50(3):475-81.

PMID: 22126376

Abstract:

Background:
There is a need for a pancreatic lipase (LIP) reference assay to provide an accurate base to which routine methods can be traceable.
Methods:
This study developed a novel LIP assay method in which 1,2-dioleoylglycerol (DODG) is the substrate and LIP activity is measured in a coupled enzymatic reaction from the increase in absorbance at 340 nm with production of NADPH.
Results:
With this method, LIP activity was linear up to 440 U/L (8-times expected upper limit of physiological concentration). When assayed manually, the between-laboratory variation for six samples surveyed at five laboratories was 3.80-26.4% (CV) for samples containing about 20-290 U/L LIP activity; when assayed using an automated analyzer, the range was 1.86-4.86% (four laboratories). Interference by >5 mmol/L glycerol and low specificity with post-heparin samples were noted, but in practice these are avoidable. Precision analyzed by automated assay of 49 samples twice in random order produced a covariance of 2.27 U/L, which is comparable to routine methods, and good correlations were obtained with five routine methods.
Conclusions:
Although further studies are required, the DODG method may be likely applicable as one candidate reference method.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25637847	Dioleoylglycerol		25637-84-7	Price

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