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1 H, 13 C and 15 N resonance assignment of human guanylate kinase

Nazimuddin Khan, David Ban, Pablo Trigo-Mourino, Marta G Carneiro, Manfred Konrad, Donghan Lee, T Michael Sabo

Biomol NMR Assign. 2018 Apr;12(1):11-14.

PMID: 28861857

Abstract:

Human guanylate kinase (hGMPK) is a critical enzyme that, in addition to phosphorylating its physiological substrate (d)GMP, catalyzes the second phosphorylation step in the conversion of anti-viral and anti-cancer nucleoside analogs to their corresponding active nucleoside analog triphosphates. Until now, a high-resolution structure of hGMPK is unavailable and thus, we studied free hGMPK by NMR and assigned the chemical shift resonances of backbone and side chain 1H, 13C, and 15N nuclei as a first step towards the enzyme's structural and mechanistic analysis with atomic resolution.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42411331 Ubiquitin-13C,15N,D human Ubiquitin-13C,15N,D human Price
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