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1-naphthyl Phosphate: Specifications for High-Quality Substrate for Measuring Prostatic Acid Phosphatase Activity

J L Bowers, G N Bowers Jr

Clin Chem. 1982 Jan;28(1):212-5.

PMID: 7198943

Abstract:

Ten lots of sodium 1-naphthyl phosphate were compared by spectrophotometry, high-performance liquid chromatography, and kinetic measurements of the activity of human prostatic acid phosphatase (EC 3.1.3.2) in serum. Two lots were readily identified as the 2-naphthyl phosphate salt by spectrophotometry and liquid chromatography. Four of the remaining eight lots of sodium 1-naphthyl phosphate met the following specifications: (a) sodium 1-naphthyl phosphate content greater than 80% by A286nm measurements (epsilon286nm1-NP = 5630 L.mol-1.cm-1) and by enzymic conversion to 1-naphthol (epsilon332nm1-N = 7560 L.mol-1.cm-1), (b) free 1-naphthol less than 3 mmol/mol, (c) inorganic phosphate less than 10 mmol/mol, and (d) the catalytic activity concentration greater than 98% maximum by absorbance assay and greater than 90% maximum by spectrofluorometric assay during simultaneous comparisons of several substrates. The need for detailed specifications and the testing of each batch of sodium 1-naphthyl phosphate is readily demonstrated by this study.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP14463684 2-Naphthyl phosphate sodium salt 2-Naphthyl phosphate sodium salt 14463-68-4 Price
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