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10-Formyl-dihydrofolic Acid Is Bioactive in Human Leukemia Cells

10-Formyl-dihydrofolic Acid Is Bioactive in Human Leukemia Cells

J E Baggott, G L Johanning

J Nutr. 1999 Jul;129(7):1315-8.

PMID: 10395592

Abstract:

The bioactivity of 10-formyl-7,8-dihydrofolic acid and 10-formyl-folic acid was determined in human leukemia (CCRF-CEM) cells grown in a folate-depleted medium containing methotrexate. Excess 10-formyl-7,8-dihydrofolic acid, (but not 10-formyl folic acid) supported the growth of these cells, but it was less potent than5-formyl-5,6,7,8-tetrahydrofolic acid (a control). 10-formyl-7, 8-dihydrofolic acid (not 10-formyl folic acid) was active as substrate for aminoimidazole carboxamide ribotide transformylase and dihydrofolate reductase. This is the first experimental evidence that 10-formyl-7,8-dihydrofolic acid is a bioactive folate in mammalian cells. These experiments and several other lines of evidence in the literature suggest that 10-formyl-folic acid must be metabolized to bioactive folate by enteric bacteria before it can be utilized by the vertebrate host.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP4033276	Dihydrofolic acid		4033-27-6	Price

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