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[15N] Leucine as a Source of [15N] Glutamate in Organotypic Cerebellar Explants

M Yudkoff, I Nissim, S Kim, D Pleasure, K Hummeler, S Segal

Biochem Biophys Res Commun. 1983 Aug 30;115(1):174-9.

PMID: 6137216

Abstract:

Approximately 26.0% of the [15N] glutamate and [alpha 15N] glutamine formed in organotypic cerebellar explants was derived from [15N] leucine. Approximately 14.0% of the 15NH3 and [amide 15N] glutamine synthesized came from leucine nitrogen. Another 4.0% of the alpha nitrogen of both glutamate and glutamine was derived from [15N] valine. These results suggest that branched-chain amino acids, particularly leucine, may be important for the synthesis of glutamic acid by the brain.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP202406528 L-Leucine-13C6,15N L-Leucine-13C6,15N 202406-52-8 Price
AP59681322 L-Glutamine-(amide-15N) L-Glutamine-(amide-15N) 59681-32-2 Price
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