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2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) Attenuates Human Platelet Aggregation, Secretion and Spreading in Vitro

2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) Attenuates Human Platelet Aggregation, Secretion and Spreading in Vitro

Ke Xiang, Gang Liu, Ya-Jun Zhou, Hong-Zhen Hao, Zhao Yin, Ao-Di He, Xing-Wen Da, Ji-Zhou Xiang, Jia-Ling Wang, Zhang-Yin Ming

Thromb Res. 2014 Feb;133(2):211-7.

PMID: 24332167

Abstract:

Introduction:
2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside(THSG) is a water-soluble component of the rhizome extract from the traditional Chinese herb Polygonum multiflorum. Recent studies have demonstrated that THSG has potent anti-oxidative and anti-inflammatory effects. In this study, we investigated the anti-platelet aggregation, secretion and spreading of THSG with different methods. The purpose was to explore the anti-platelet effect of THSG and the underlying mechanism.
Materials and methods:
We investigated the anti-platelet activity of THSG on platelet aggregation induced by collagen (2 μg/mL), thrombin(0.04U/mL), U46619 (3 μM) and ADP (2 μM). ATP secretion induced by collagen (2 μg/mL) was also investigated. P-selectin expression and PAC-1 binding were measured by flow cytometry. In addition, human platelet spreading on immobilized fibrinogen and immunoblotting were also tested.
Results:
THSG dose-dependently inhibited platelet aggregation and ATP secretion induced by collagen. It inhibited platelet P-selectin expression and PAC-1 binding induced by thrombin(0.1U/mL). THSG also inhibited human platelet spreading on immobilized fibrinogen, a process mediated by platelet outside-in signaling. Western blot analysis showed that THSG could inhibit platelet Fc γ RIIa, Akt(Ser473)and GSK3β(Ser9) phosphorylation.
Conclusions:
Our study indicates that THSG has potent anti-platelet activity to collagen induced aggregation. THSG is likely to exert protective effects in platelet-associated thromboembolic disorders by modulating human platelet.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP82373942	2,3,5,4′-Tetrahydroxystilbene 2-O-β-D-glucoside		82373-94-2	Price

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