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2-(5-Benzoacridine)ethyl-p-toluenesulfonate as Sensitive Reagent for the Determination of Bile Acids by HPLC With Fluorescence Detection and Online Atmospheric Chemical Ionization-Mass Spectrometric Identification

2-(5-Benzoacridine)ethyl-p-toluenesulfonate as Sensitive Reagent for the Determination of Bile Acids by HPLC With Fluorescence Detection and Online Atmospheric Chemical Ionization-Mass Spectrometric Identification

Jinmao You, Yanyan Fu, Zhiwei Sun, Yourui Suo

Anal Bioanal Chem. 2010 Apr;396(7):2657-66.

PMID: 20140426

Abstract:

2-(5-Benzoacridine)ethyl-p-toluenesulfonate (BAETS), a dual-sensitive probe, was reacted with bile acids in the presence of K(2)CO(3) catalyst in dimethyl sulfoxide (DMSO) solvent to give BAETS-bile acid derivatives. Derivatives exhibited intense fluorescence (FL) with an excitation maximum at lambda(ex) 270 nm and an emission maximum at lambda(em) 510 nm. MS analysis using APCI-MS indicated that derivatives had excellent APCI-MS ionizability with percentage ionization delta values changing from 0 to 88.83% in aqueous acetonitrile and from 0 to 89.15% in aqueous methanol. The collision induced dissociation spectra of m/z [M + H](+) contained specific fragment ions at m/z [M + H-H(2)O](+), [M + H-2H(2)O](+), [M + H-3H(2)O](+), 347.3, and 290.1. Repeatability was good for LC separation of BAETS-bile acid derivatives with aqueous acetonitrile as mobile phase. The relative standard deviations (RSDs) of retention time and peak area at 6.6 nmol mL(-1) levels with fluorescence detection (FL) were from 0.045 to 0.072% and from 2.16 to 2.73%, respectively. Excellent linear responses were observed, with regression coefficients >0.9995. The FL detection limits (S/N = 3) were in the range of 18.0-36.1 fmol. The online APCI-MS detection limits are in the range of 500-790 fmol (at a signal-to-noise ratio of 3).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP80400	Ethyl p-toluenesulfonate		80-40-0	Price

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