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2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein as a Dual-Emission Fluorescent Indicator of Intracellular pH Suitable for Argon Laser Confocal Microscopy

2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein as a Dual-Emission Fluorescent Indicator of Intracellular pH Suitable for Argon Laser Confocal Microscopy

E Lanz, J Slavík, A Kotyk

Folia Microbiol (Praha). 1999;44(4):429-34.

PMID: 10983238

Abstract:

The widely used fluorescent probe 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) serves as a pH-sensitive indicator in classical microscopy. Characteristics of BCECF were studied and a way of employing the probe in a confocal laser scanning microscope equipped with an argon laser at 488 nm was developed, based on the fact that the emission fluorescence spectra are pH-dependent with spectral maximum shift from 518 to 529 nm. Optical filters for the dual-emission ratio method were set to 506 and 529 nm. pH values measured inside a single cell of Saccharomyces cerevisiae were similar to those obtained with other pH estimation methods.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP85138494	2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein		85138-49-4	Price

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