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2-(Benzothiazol-2-yl)-phenyl-β-d-galactopyranoside Derivatives as Fluorescent Pigment Dyeing Substrates and Their Application for the Assay of β-d-galactosidase Activities

2-(Benzothiazol-2-yl)-phenyl-β-d-galactopyranoside Derivatives as Fluorescent Pigment Dyeing Substrates and Their Application for the Assay of β-d-galactosidase Activities

Tadamune Otsubo, Akira Minami, Haruna Fujii, Risa Taguchi, Tadanobu Takahashi, Takashi Suzuki, Fumiteru Teraoka, Kiyoshi Ikeda

Bioorg Med Chem Lett. 2013 Apr 1;23(7):2245-9.

PMID: 23434417

Abstract:

2-(Benzothiazol-2-yl)-phenyl-β-d-galactopyranoside derivatives were synthesized as novel artificial fluorescent pigment dyeing substrates for β-d-galactosidase. The substrates, which exhibited non-fluorescence or weak fluorescence in solution phase, were smoothly hydrolyzed by β-d-galactosidase from Aspergillus oryzae and yielded a water-insoluble strong fluorescent pigment. The difference of fluorescent intensity exhibited a linear relationship with the amount of enzyme.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2818588	Phenyl-β-D-galactopyranoside		2818-58-8	Price

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