Home
Resources
Publications
3,4-dihydroxy-L-phenylalanine as a Cell Adhesion Molecule in Serum-Free Cell Culture

3,4-dihydroxy-L-phenylalanine as a Cell Adhesion Molecule in Serum-Free Cell Culture

Wan-Geun La, Suk Ho Bhang, Jung-Youn Shin, Hee Hun Yoon, Jooyeon Park, Hee Seok Yang, Seung-Ho Yu, Yung-Eun Sung, Byung-Soo Kim

Biotechnol Prog. 2012 Jul;28(4):1055-60.

PMID: 22581651

Abstract:

In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP59927	3,4-Dihydroxy-L-phenylalanine		59-92-7	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: